a novel high performance liquid chromatographic method for determination of nystatin in pharmaceutical formulations by box–behnken statistical experiment design
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abstract
this study a novel high performance liquid chromatography for the assay of nystatin in oral and vaginal tablets were optimized and validated using box–behnken experimental design. the method was performed in the isocratic mode on a rp-18 column (30 °c) using a mobile phase consisting of 0.05 m ammonium acetate buffer/ methanol mixture (30:70) and a flow-rate of 1.0 ml/min. the method was validated by specificity, linearity, precision, accuracy, lod and loq. the method was linear over the range of 5–500 µg/ml with a good correlation coefficient (r2 = 0.9996). the limit of detection (lod) and quantification (loq) were 0.01 and 0.025 µg/ml respectively. the obtained results clearly indicate that the proposed validated method can be used as an alternative method for assay of nystatin.
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A Novel High Performance Liquid Chromatographic Method for Determination of Nystatin in Pharmaceutical Formulations by Box–Behnken Statistical Experiment Design
this study a novel High Performance Liquid Chromatography for the assay of nystatin in oral and vaginal tablets were optimized and validated using Box–Behnken experimental design. The method was performed in the isocratic mode on a RP-18 column (30 °C) using a mobile phase consisting of 0.05 M ammonium acetate buffer/ Methanol mixture (30:70) and a flow-rate of 1.0 ml/min. The method was valida...
full textA Novel High Performance Liquid Chromatographic Method for Determination of Nystatin in Pharmaceutical Formulations by Box–Behnken Statistical Experiment Design
this study a novel High Performance Liquid Chromatography for the assay of nystatin in oral and vaginal tablets were optimized and validated using Box–Behnken experimental design. The method was performed in the isocratic mode on a RP-18 column (30 °C) using a mobile phase consisting of 0.05 M ammonium acetate buffer/ Methanol mixture (30:70) and a flow-rate of 1.0 ml/min. The method was valida...
full textA Novel High Performance Liquid Chromatographic Method for Determination of Nystatin in Pharmaceutical Formulations by Box–Behnken Statistical Experiment Design
In this study a novel High Performance Liquid Chromatography for the assay of nystatin in oral and vaginal tablets were optimized and validated using Box-Behnken experimental design. The method was performed in the isocratic mode on a RP-18 column (30 °C) using a mobile phase consisting of ammonium acetate 0.05 M buffer/ Methanol mixture (30:70) and a flow-rate of 1.0 mL/min. The specificity, l...
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full textdevelopment and validation of high performance liquid chromatography for determination of nystatin in pharmaceutical formulations
full text
Liquid chromatographic determination of nystatin in pharmaceutical preparations.
A rapid, reversed-phase liquid chromatographic method was developed for the assay of nystatin in the bulk drug and a variety of dosage forms. Analysis was performed on a Symmetry C18 reversed-phase column using a mobile phase of methanol-water-dimethylformamide (DMF; 55 + 30 + 15, v/v/v), with detection by UV at 305 nm. Quantitation is based on the sum of the peak areas of the 2 major isomers o...
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Journal title:
iranian journal of pharmaceutical researchجلد ۱۴، شماره supplement، صفحات ۴۳-۴۹
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